Molecular Biology and Genetics help

[Superfoot]

I hope this within the rules....

Evening everyone. I'm studying for an item of coursework, which is due to be handed in quite some time away from now, but I thought it best to start early.

I'm having a bit of trouble on a certain question. I am by no means asking for you to do my work for me, because right now, Im doing that myself, albeit with great difficulty. And so I turn to you.

My question. Can anyone describe a procedure for obtaining a FULL Length cDNA (Complementary DNA) clone for the supposed novel "Opsin", which is expressed in Xenopus skin.

Xenopus is a frog species.

Thats all for now, Im still reading my book and notes, but no answers. If you could help me I would be most grateful. No, I shan't plagiarise. Also, any links to such methods would be helpful too. I can't seem to find much on Wikipedia or the rest of the web.

Thanx

[Why_Worry]

I dont really know, but it seems liek more people comment when someone has already commented so i figured i woudl just comment and good luck figuring it out. You could always ask the teacher though if youc ant figure it out.

[Rich_2k3]

Are you doing A-levels or a Degree? I just got my A-level Biology results (got a C), that doesnt look familiar to me, although I could look it up in my study books.

[Superfoot]

I'm doing a degree Rich, but thanx. Actually, my lecturer has extended the deadline for all of us, so not much worry is on my hands. Congrats on your results. What exam board did you do for Bio. I did OCR.

I have some idea on how to make a clone. But not how to obtain one. However, clones can be cloned from other clones.

So here's what I thunk so far.

Using Adapter Oligonucleotides, I can perform Blunt-end Ligation on cDNA.

As a result of this I get a new product of modified cDNA, which I can ligate onto a prepared vector to give me a recombinant plasmid.

[Rich_2k3]

That explains it then, thought that looked abit advanced for A-level oh and it was AQA. Taking a gap year at the moment, gonna go travelling in thailand for a few months, cant wait gonna be studying criminology next year.

[Jay]

im doing as biologie at the moment shame i cant help you im better at physics your trying to find out the procedure of cloning a frog skin???

[Rich_2k3]

Lets hope they dont ask you how to spell biology in your exam

[Superfoot]

Heh! I think these days, you get two marks for filling your name in correctly. AQA I heard was exceptionally hard, so props to you for not dropping it at AS level, and good luck with the criminology.

Biology and Physics together Jay? That's very brave. They wouldnt let me take all sciences at A level at my college. They said that when universities look at my personal statement, they wouldnt like to see a "bookworm". Sob...

Actually the frog looks pretty cool in the textbook. It has claws an good camouflage. The amphibian Yautja. I'm still searching for extra details regarding cloning, but all is going well.